FastQCFastQC Report
Tue 10 Nov 2015
MethylC-Seq_mm_fc_1wk_SRR921767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMethylC-Seq_mm_fc_1wk_SRR921767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000000
Sequences flagged as poor quality0
Sequence length100
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA301.455961E-678.343281
AACCCCG200.002164732470.4900894
TCTTCCG1450.068.064195
CTCTTCC1550.066.704434
GCTCTTC1550.066.704433
ACGCTCT1550.063.68551
CTTCCGA1600.061.6786426
CGCTCTT1650.059.8134772
TCCTAGG250.00523880656.396055
AACTCCT654.814501E-850.6082794
CTCCAGC1201.8189894E-1243.07727494
TCCGATC2750.042.7326971
CCGATCT2950.039.8273772
CAGCTAT500.00170166637.6047741
CCAGTCC1055.125912E-835.8044994
CACCTCT550.00271999534.17701394
TCAACTT600.004164279431.3308683
CGATCTT2500.030.0776353
TTCCGAT3750.028.8225277
ACTCCAT1154.205738E-628.60467394
Produced by FastQC (version 0.11.2)