Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_1wk_SRR921769_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 20400069
Single-end alignments with a unique best hit 17482188
Sequences without alignments under any condition 717541
Sequences that did not map uniquely 2200340
Genomic sequence context not extractable (edges of chromosomes) 4

Cytosine Methylation

Total C's analysed 326018397
Methylated C's in CpG context 9840690
Methylated C's in CHG context 382486
Methylated C's in CHH context 1349951
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 2899152
Unmethylated C's in CHG context 68965476
Unmethylated C's in CHH context 242580642
Unmethylated C's in Unknown context 30
Percentage methylation (CpG context) 77.2%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8770492 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 8711692 original bottom strand

Deduplication

Alignments analysed 17482184
Unique alignments 17194498
Duplicates removed 287686
Duplicated alignments were found at 74823 different positions

Cytosine Methylation after Extraction

Total C's analysed 321444403
Methylated C's in CpG context 9279423
Methylated C's in CHG context 374888
Methylated C's in CHH context 1309578
Unmethylated C's in CpG context 2771305
Unmethylated C's in CHG context 68115589
Unmethylated C's in CHH context 239593620
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot