FastQCFastQC Report
Sat 20 Feb 2016
P4107_1005_S5_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP4107_1005_S5_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484233426
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG21067140.4350616638348299TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4434450.080.478311
TCGGAAG4603950.077.162032
CGGAAGA4847750.073.256913
AGAGCAC7545250.047.298527
AGCACAC7859350.045.4064149
GAGCACA8051200.044.352348
GAAGAGC8274200.043.2191245
AAGAGCA10782450.033.339456
GGAAGAG12088700.029.9210134
TATGCCG3277750.022.89552745-49
CTCGTAT3129350.021.98740640-44
TCTCGTA3154600.021.74840-44
CGTATGC3421700.021.69800445-49
TCGTATC3482450.021.54702635-39
ATGCCGT3537150.021.19514845-49
TATCTCG3248900.020.87133840-44
GCCGTCT3601000.020.4771450-54
ATCTCGT3415800.020.11509940-44
ATTCGTA3727550.019.96804635-39
TGCCGTC3554500.019.96266745-49