Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_1wk_SRR921768_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 99937484
Single-end alignments with a unique best hit 85690327
Sequences without alignments under any condition 3389442
Sequences that did not map uniquely 10857715
Genomic sequence context not extractable (edges of chromosomes) 39

Cytosine Methylation

Total C's analysed 1601467168
Methylated C's in CpG context 48152573
Methylated C's in CHG context 1861779
Methylated C's in CHH context 6584752
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 14292014
Unmethylated C's in CHG context 338854830
Unmethylated C's in CHH context 1191721220
Unmethylated C's in Unknown context 106
Percentage methylation (CpG context) 77.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 42966351 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 42723937 original bottom strand

Deduplication

Alignments analysed 85690288
Unique alignments 83205980
Duplicates removed 2484308
Duplicated alignments were found at 1280568 different positions

Cytosine Methylation after Extraction

Total C's analysed 1559936769
Methylated C's in CpG context 44808244
Methylated C's in CHG context 1801911
Methylated C's in CHH context 6303584
Unmethylated C's in CpG context 13403348
Unmethylated C's in CHG context 330756121
Unmethylated C's in CHH context 1162863561
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot