Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_2wk_SRR921694_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 153092768
Single-end alignments with a unique best hit 120059544
Sequences without alignments under any condition 12078510
Sequences that did not map uniquely 20954714
Genomic sequence context not extractable (edges of chromosomes) 19

Cytosine Methylation

Total C's analysed 2050033670
Methylated C's in CpG context 88264379
Methylated C's in CHG context 4976648
Methylated C's in CHH context 16015321
Methylated C's in Unknown context 7
Unmethylated C's in CpG context 27338288
Unmethylated C's in CHG context 528140316
Unmethylated C's in CHH context 1385298718
Unmethylated C's in Unknown context 240
Percentage methylation (CpG context) 76.4%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 60747577 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 59311948 original bottom strand

Deduplication

Alignments analysed 120059525
Unique alignments 111688896
Duplicates removed 8370629
Duplicated alignments were found at 3449037 different positions

Cytosine Methylation after Extraction

Total C's analysed 1928202931
Methylated C's in CpG context 74456543
Methylated C's in CHG context 4731636
Methylated C's in CHH context 14906573
Unmethylated C's in CpG context 23848801
Unmethylated C's in CHG context 500040303
Unmethylated C's in CHH context 1310219075
Percentage methylation (CpG context) 75.7%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%

M-Bias Plot