Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_2wk_SRR921773_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 128125879
Single-end alignments with a unique best hit 97745852
Sequences without alignments under any condition 11557697
Sequences that did not map uniquely 18822330
Genomic sequence context not extractable (edges of chromosomes) 4

Cytosine Methylation

Total C's analysed 1556887362
Methylated C's in CpG context 64856205
Methylated C's in CHG context 3746414
Methylated C's in CHH context 12200224
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 20074609
Unmethylated C's in CHG context 394817230
Unmethylated C's in CHH context 1061192680
Unmethylated C's in Unknown context 276
Percentage methylation (CpG context) 76.4%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 49415480 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 48330368 original bottom strand

Deduplication

Alignments analysed 97745848
Unique alignments 91566412
Duplicates removed 6179436
Duplicated alignments were found at 2369116 different positions

Cytosine Methylation after Extraction

Total C's analysed 1472451692
Methylated C's in CpG context 55160868
Methylated C's in CHG context 3583627
Methylated C's in CHH context 11458218
Unmethylated C's in CpG context 17617658
Unmethylated C's in CHG context 375850399
Unmethylated C's in CHH context 1008780922
Percentage methylation (CpG context) 75.8%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%

M-Bias Plot