Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_1wk_SRR921767_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 99942367
Single-end alignments with a unique best hit 85970837
Sequences without alignments under any condition 3096960
Sequences that did not map uniquely 10874570
Genomic sequence context not extractable (edges of chromosomes) 40

Cytosine Methylation

Total C's analysed 1609945962
Methylated C's in CpG context 48410817
Methylated C's in CHG context 1872919
Methylated C's in CHH context 6627385
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 14337271
Unmethylated C's in CHG context 340247268
Unmethylated C's in CHH context 1198450302
Unmethylated C's in Unknown context 88
Percentage methylation (CpG context) 77.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 43105354 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 42865443 original bottom strand

Deduplication

Alignments analysed 85970797
Unique alignments 83465166
Duplicates removed 2505631
Duplicated alignments were found at 1292989 different positions

Cytosine Methylation after Extraction

Total C's analysed 1567978762
Methylated C's in CpG context 45027781
Methylated C's in CHG context 1811762
Methylated C's in CHH context 6342884
Unmethylated C's in CpG context 13443163
Unmethylated C's in CHG context 332074146
Unmethylated C's in CHH context 1169279026
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot