Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_2wk_SRR921695_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 153372033
Single-end alignments with a unique best hit 120235451
Sequences without alignments under any condition 12132257
Sequences that did not map uniquely 21004325
Genomic sequence context not extractable (edges of chromosomes) 10

Cytosine Methylation

Total C's analysed 2052042902
Methylated C's in CpG context 88328643
Methylated C's in CHG context 4983440
Methylated C's in CHH context 16036856
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 27349290
Unmethylated C's in CHG context 528630157
Unmethylated C's in CHH context 1386714516
Unmethylated C's in Unknown context 249
Percentage methylation (CpG context) 76.4%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 60833866 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 59401575 original bottom strand

Deduplication

Alignments analysed 120235441
Unique alignments 111849677
Duplicates removed 8385764
Duplicated alignments were found at 3457979 different positions

Cytosine Methylation after Extraction

Total C's analysed 1930076424
Methylated C's in CpG context 74520126
Methylated C's in CHG context 4738195
Methylated C's in CHH context 14929689
Unmethylated C's in CpG context 23854898
Unmethylated C's in CHG context 500496609
Unmethylated C's in CHH context 1311536907
Percentage methylation (CpG context) 75.8%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.1%

M-Bias Plot