Bismark

Bismark Processing Report

MethylC-Seq_mm_fc_1wk_SRR921770_1_trimmed.fq.gz

Data processed at 09:00 on 2015-11-11


Alignment

Sequences analysed in total 99944444
Single-end alignments with a unique best hit 85880328
Sequences without alignments under any condition 3179359
Sequences that did not map uniquely 10884757
Genomic sequence context not extractable (edges of chromosomes) 26

Cytosine Methylation

Total C's analysed 1604547987
Methylated C's in CpG context 48204032
Methylated C's in CHG context 1872979
Methylated C's in CHH context 6602284
Methylated C's in Unknown context 1
Unmethylated C's in CpG context 14298683
Unmethylated C's in CHG context 339441953
Unmethylated C's in CHH context 1194128056
Unmethylated C's in Unknown context 76
Percentage methylation (CpG context) 77.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 43061055 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 42819247 original bottom strand

Deduplication

Alignments analysed 85880302
Unique alignments 83381921
Duplicates removed 2498381
Duplicated alignments were found at 1279231 different positions

Cytosine Methylation after Extraction

Total C's analysed 1562840273
Methylated C's in CpG context 44827612
Methylated C's in CHG context 1812592
Methylated C's in CHH context 6320794
Unmethylated C's in CpG context 13404041
Unmethylated C's in CHG context 331308033
Unmethylated C's in CHH context 1165167201
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot